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Trichomonas vaginalis, NAA

Trichomonas vaginalis, NAA

Test ID: A932

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Trichomoniasis is a common but curable sexually transmitted disease (STD) caused by Trichomonas vaginalis. Although only 30% of those infected with “Trich” develop symptoms, it can still be transmitted to sexual partners and increases the risk of contracting other STDs, including HIV. Early detection enables prompt treatment to prevent transmission and health complications.



How to order a test


  • Trichomonas vaginalis nucleic acid amplification (NAA) test
  • Trichomoniasis Test

What is Included?

Detection of Trichomonas vaginalis by nucleic acid amplification technology

Turnaround Time

1 – 3 business days
The turnaround time is not guaranteed. The average turnaround time is 1 – 3 business days from the date that the sample arrives at the laboratory. Shipping time for the sample is not included. Additional time is required if the case requires confirmatory or reflex testing, or if the sample is insufficient, or if a recollection is required.

Related Documents

Additional Information and Resources

Special Instructions

Do not apply the transport medium directly to skin or mucous membranes or take internally.


Preparation Before Specimen Collection

  • Do not urinate for at least one hour prior to urine collection.
  • Females should not cleanse the labial area prior to urine collection.

  • Antibiotics can affect this test so wait until at least seven days after completing a course of antibiotics before collecting urine sample.

Specimen Type

First-void urine
Urine sample collected from the initial urine stream at least one hour after previous urination.


2 mL from a 20 – 30 mL collection of first-void urine.
Repeat urine collection (at least 1 hour after previous urination) if more than 60ml of first-void urine is collected.

Minimum volume

2 mL


Urine specimen transport container

Collection Method

This test requires a first-void urine specimen. Wait at least 1 hour after previous urination before collecting the first-void urine. All supplies for sample collection are provided in this kit.

  1. Collect 20 – 30 mL from the initial urine stream into the sterile urine collection container. Collection of larger volumes of urine may result in specimen dilution that may reduce test sensitivity; lesser volumes may not adequately rinse organisms into the specimen.
  2. Transfer 2 mL of urine into the urine specimen transport container using the disposable pipette provided. Transfer to the urine specimen transport container must occur within 24 hours of collection, and liquid level must fall between the two black indicator lines on the tube label.
  3. Re-cap the urine specimen transport container tightly. Seal in the transport bag and return to the laboratory in the provided prepaid return-shipping envelope.

Specimen Storage

Maintain specimen at temperatures between 2°C and 30°C during storage and transport.

Specimen Stability

Specimens stored at temperatures between 2°C and 30°C must be tested within 30 days of collection. If longer storage is required, urine specimens may be frozen in the urine specimen transport container within 7 days of collection and tested within 12 month of collection.

Causes for Rejection

  • Incorrect or incomplete patient identification
  • Incorrect specimen collection
  • Inappropriate storage and transport conditions
  • Loss of urine specimen through container leakage
  • Incorrect specimen volume
  • Specimen received >30 days after collection



To detect Trichomonas vaginalis nucleic acid in a urine specimen


  • This report is not intended for use in medico-legal applications.
  • These results are intended for screening and monitoring for trichomoniasis, and should be interpreted in conjunction with other laboratory and clinical information.
  • Correct specimen collection and handling is required for optimal assay performance.
  • The effects of tampon use, douching, and specimen collection variables have not been assessed for their impact on the detection of Trichomonas vaginalis.
  • A negative result does not exclude the possibility of infection. False-negative test results may occur due to improper specimen collection, concurrent antibiotic therapy, presence of inhibitors, or organism levels below the sensitivity of this assay (which is common within 2 weeks post-exposure).
  • The presence of Trichomonas tenax or Pentatrichomonas hominis in a specimen may also affect the ability to detect Trichomonas vaginalis rRNA.
  • False-positive results are rare, but may be more frequent in low-prevalence populations. A false-positive result may also occur directly after successful antimicrobial therapy, as Trichomonas vaginalis nucleic acid may persist for 3 weeks or more.
  • The performance of this assay has not been evaluated in adolescents less than 14 years of age. 


Nucleic acid amplification


No Trichomonas vaginalis detected